This study aimed to improve radiation safety behaviors in veterinary workers through a tailored training module and to identify factors influencing radiation safety practices. The research included two components: a prospective observational study and a survey. The observational study involved veterinary workers performing radiographs on small animals. A 7-minute training video focused on proper use of personal protective equipment (PPE) and radiation safety behaviors. Observations before and after the training showed a significant increase in the correct use of leaded eyeglasses and gloves, as well as improved radiation safety behaviors, such as optimal body and head positioning during radiography. The survey, distributed to veterinary specialists, revealed that workers with better knowledge of ionizing radiation risks and safety guidelines were more likely to use PPE and adjust machine settings to reduce exposure. The study concludes that ongoing education, clearer policies, and broader dissemination of training materials could further improve safety behaviors in veterinary radiation settings.